Part:BBa_K1403019

Paris Bettencourt 2014 Odor Palette

Our odor palette is composed of BioBricks containing codon-optimized sequences of enzymes known to catalyze reactions that yield volatile compounds with distinctive smells.

We included odors with different tonalities in order to explore the aromas resulting from combinations of smelly units in E. coli. The tonality of each smell is categorized as butter, balminess, citrus, non-citrus fruit and herbal. These cover half of the main odor categories perceivable to human beings.

Each part has a standardized architecture consisting on a constitutive promoter and a functional RBS. The palette could be used as an educational tool to introduce synthetic biology concepts to the general public.

System	Enzyme	Substrate	Concentration	Product
Jasmine BBa_K1403006	JMT	Jasmonic acid	1 mM	Methyl jasmonate
Mint BBa_K1403009	BMST1	Salicylic acid	2 mM	Methyl salicylate
Banana BBa_K1403012	ATF1	Isoamyl alcohol	5 mM	Isoamyl acetate
Limonene BBa_K1403003	LIMS1	Farnesyl diphosphate	Natural	(+)-Limonene
Rain BBa_K1403013	GDS	Farnesyl diphosphate	Natural	Geosmin
Flowers BBa_K1403009	BMST1	Benzoic acid	5 mM	Methyl benzoate
Butter BBa_K1403017 & BBa_K1403019	AldB ilvB/N	Acetolactic acid Pyruvate	Natural	Acetoin
Sweat BBa_K1403002	agaA	3-M-2-hexenoic acid	-	3-HO-3-Methylhexanoic acid

Acetohydroxybutanoate synthase /acetolactate synthase (ilvB/N) expression cassette

• Constitutive T7 promoter
• RBS
• CDS: IlvB/N from E. coli MG1655

Transform E. coli to overexpress ilvB and thus increase the basal production of alpha acetolactate, the precursor of acetoin.

• This was submitted in a pETDuet-1
  

Click on Design tab for more information.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 47
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 47
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 47
  
• 1000
  COMPATIBLE WITH RFC[1000]